FIGURE 1.
PatMC enhances Lsm1–7 binding to adenine-rich substrates in a cooperative manner. (A) Schematic of Pat1 domains and Lsm1–7. (B) SDS-PAGE of the Lsm1–7 complex alone (left) or with PatMC (right). Molecular weight (kDa) shown on right. (C) Lsm1–7 or PatMC/Lsm1–7 binding to different 5′-FAM labeled 15mer RNAs (A15, A14U1, A10U5, U5A10, U10A5, U15) monitored by fluorescence polarization. Numbers correspond to labels in (D). Binding affinities were converted to ΔG and plotted against the fractional uracil content of the oligonucleotides (n = 3). (D) Hill coefficients for each fit for the binding isotherms shown in C (n = 3).
