Prefoldin-like Bud27 influences the transcription of ribosomal components and ribosome biogenesis in Saccharomyces cerevisiae

The phosphorylation status of S6 is altered in the cells lacking Bud27. Western blot analysis of the phosphorylated (Rps6-P) and nonphosphorylated (Rps6) S6 RP in both the wild-type and bud27Δ mutant cells. Cells were grown in YPD medium at 30°C (time 0) or in YPD containing 400 ng/mL rapamycin for the indicated times. Anti-phospho (pSer235/236)-S6 RP and anti-S6 RP antibodies were used. Pgk1 was taken as the internal control.