Prefoldin-like Bud27 influences the transcription of ribosomal components and ribosome biogenesis in Saccharomyces cerevisiae

Absence of Bud27 impairs translation and 60S r-subunit metabolism. Polysomes profile analysis of the wild-type and bud27Δ cells grown to the mid-log phase in YPD medium at 30°C and then shifted to 37°C for 6 h. Cells were harvested at an OD₆₀₀ of about 0.8. Whole-cell extracts were prepared and 10 A₂₆₀ units of each extract were resolved in 7%–50% sucrose gradients. A₂₅₄ was continuously measured. Sedimentation goes from left to right. The peaks of the free 40S and 60S r-subunits, 80S vacant ribosomes or monosomes and polysomes, are indicated.