FIGURE 7.
De-repression of pho1 by erh1Δ depends on DSR elements in the prt lncRNA. The top panel shows a schematic of the prt–pho1 locus in the reporter plasmid that highlights the two clusters of triplet DSR elements recognized by Mmi1. The nucleotide sequence of the wild-type DSR clusters and the base mutations introduced into the mut1 and mut2 variants are shown below the graph. The reporter plasmids with wild-type or mutated DSRs were transfected into erh1+ (WT) or erh1Δ strains in which the chromosomal pho1 locus was deleted. Acid phosphatase activity was determined as described in Figure 3D.
