FIGURE 4.
De-repression of pho1 expression by erh1Δ depends on CPF subunits and Rhn1. (A) S. pombe strains with the genotypes indicated at left were inoculated in YES broth and grown at 30°C. Exponentially growing cultures were adjusted to A600 of 0.1, and aliquots (3 µL) of serial fivefold dilutions were spotted on YES agar and then incubated at the temperatures specified. (B) S. pombe strains bearing the indicated erh1 alleles (wild-type or erh1Δ) in combination with CPF subunit or Rhn1 mutations as specified were grown in liquid culture at 30°C and assayed for acid phosphatase activity.
