Does erh1Δ affect the prt lncRNA or pho1 mRNA promoters? (A) Schematic of the plasmid-borne prt–pho1 reporter in which pho1 expression is repressed by prt lncRNA transcription. (B) A reporter of pho1 promoter activity in which prt lncRNA transcription is abolished by mutations (indicated by X) in the HomolD and TATA box elements in the prt promoter (Chatterjee et al. 2016). (C) A reporter of prt promoter activity in which the prt promoter directly drives transcription of the pho1 gene. (D–F) The indicated reporter plasmids were transfected into erh1+ (WT) or erh1Δ strains in which the chromosomal pho1 locus was deleted. Transformants were selected and single colonies of individual transformants were pooled (>20) and grown in plasmid-selective liquid medium to A600 of 0.5–0.8. Aliquots were harvested for acid phosphatase activity measurements. Each datum in the bar graph is the average of assays using cells from three independent cultures ± SEM.
