Expression of classical master regulators and cell surface markers across T-cell subsets. Expression of signature genes typically used to delineate T Helper cells, including genes encoding (A) master transcription regulators and (B) cell-surface proteins, across all data sets. Each subplot represents a distinct gene, while each column in a specific subplot is a distinct study. Studies are listed at bottom and ordered as in Figure 1A. Cell type listed on right is the subtype expected to be enriched for the two genes plotted on that row. For each study, the mean and distribution of the given gene is displayed as a box plot, with the values for each individual sample represented as a dot. Dots are colored by cell subtype. Note that not all studies contain all cell types, as described in Figure 1A. Expression values shown as the inverse hyperbolic sine (asinh) of transcripts per million (TPM). (C) Comparison of the median TPM in the expression of the indicated genes in the corresponding cell subtype relative to all others in the data set. Dots are values for individual data sets, and the boxplot shows the range and median for each gene. In each case, the darker color is the master regulator and the lighter color is the surface marker.
