FIGURE 6.
The TLR3-NF-κB pathway is inhibited by miR-146a in CVB3 infection. HeLa cells were treated with AMO-146a and scramble RNA, respectively. These cells were infected with CVB3 12 h posttransfection. (A) Total, cytoplasmic, and nuclear proteins were extracted 24 h p.i. The p65 subunit of NF-κB was detected with western blotting. The cytoplasmic fraction was normalized by β-actin, and the nuclear fraction was normalized by histidine H3. (B,C) The mRNA abundance of IL-6 and TNF-α were quantified by RT-qPCR. Error bars represent SD, n = 3.
