SNORD113 N-Mid contains antisense RNA. (A) Native PAGE comparison of dsSNORD113 (generated by annealing synthetic sense and synthetic antisense RNA) to in vitro transcribed middle band (IVT N-Mid). A total of 200 nM of each RNA was subjected to 8% native PAGE and visualized with SYBR Gold. (B) RNA samples (500 ng) were separated by denaturing PAGE and subjected to a northern blot analysis with a 32-P-labeled probe designed to detect antisense SNORD113. A representative blot (n = 3, two biological replicates with an additional technical replicate) is shown. (Lane 1) Labeled RNA decade marker (Thermo Fisher); (lane 2) synthetic sense SNORD113 RNA; (lane 3) synthetic antisense SNORD113 RNA; (lane 4) dsSNORD113; (lane 5) empty; (lane 6) in vitro transcribed SNORD113 N-Mid. Red asterisk indicates detection of antisense SNORD113 in the N-Mid lane.
