Minor SNORD113 species is a potent PKR activator. SNORD113 species were gel purified after 8% denaturing-PAGE or 8% native-PAGE prior to the following experiments. (A) Representative PhosphorImage of an autophosphorylation assay with SNORD113 Den, N-Top, N-Bot at 10 nM, 100 nM, 500 nM; N-Mid 10 nM, 100 nM, 250 nM; ds106, 10 nM. (B) 8% native-PAGE analysis of SNORD113 species. (C) Averaged activation data as assayed in A. Error bars ± SD; n = 3 with at least two biological replicates. We believe the large error bars reflect differing amounts of contaminating antisense RNA produced by T7 RNAP in different in vitro transcription reactions. At present we do not know the parameters that affect this variable.
