FIGURE 4.
Sedimentation velocity analytical ultracentrifugation (SV-AUC) analyses of SNORD113. (A) Sedimentation coefficient c(s) distribution analysis of SNORD113 conformers. [RNA] was 0.5 µM in AU200 buffer. Peaks corresponding to monomer, dimer, and >dimer species of SNORD113 are indicated. (B) Native PAGE analysis of SNORD113 recovered after SV-AUC. RNA was visualized with SYBR Gold. Below the gel are the amounts of SNORD113 monomer, dimer, and >dimer as determined by SV-AUC; the average PKR activation by each RNA sample is indicated in the bottom row. The SV-AUC results from two additional SNORD113 purifications are shown in Supplemental Tables S1 and S2.
