FIGURE 2.
PKR is activated by in vitro transcribed SNORD113 after phosphatase treatment. SNORD113 was initially purified by denaturing PAGE followed by the indicated phosphatase treatments. (A) Representative PhosphorImage of an autophosphorylation assay with in vitro transcribed SNORD113 treated without (mock) or with the indicated phosphatase; (CIP) calf intestinal phosphatase, (AnP) antarctic phosphatase, (RppH) RNA 5′ pyrophosphohydrolase. SNORD113, 100 nM, 500 nM, 1000 nM; ds106, 10 nM. (B) Activation data as assayed in A were normalized to ds106 and averaged values were plotted. Error bars ± SD; n = 3 with at least two biological replicates.
