Visualization of MRB10130-containing complexes using glycerol gradient sedimentation. (A) Stepwise description of the method used to isolate and visualize the MRB10130-containing complexes. (B) MRB10130-PTP was affinity purified from PF T. brucei and then subjected to 10%–30% (v/v) glycerol gradient sedimentation. Fractions collected were analyzed by western blot with antibodies against RESC proteins. Size markers obtained from a parallel gradient are displayed below the blots. Shown are representative western blots of two biological replicate experiments. (C) Total cell lysate from PF T. brucei containing the MRB10130-PTP construct was subject to 10%–30% (v/v) glycerol gradient sedimentation. Fractions were collected and analyzed by western blot. Shown is a representative of two biological replicate experiments.
