Validation of DASEs regulated by PRPF40B. (A) Validation by RT-PCR of 16 selected DASEs altered by PRPF40B-KO and rescued by WT protein. All DASEs in all panels are cassette exons except the indicated IR. Upper bars depict the RNA-seq–derived PSIs (scale 0–1), while bottom numbers indicate PSIs (scale 0–100) by RT-PCR quantification (PSIs are not identical because PCR biases against larger isoforms). (B) RT-PCR validation of eight selected DASEs not rescued by PRPF40B-WT. (C, right table) For each tested DASE gene, we indicate the correlation coefficient between the PSIs by RNA-seq and RT-PCR for the seven conditions. Smaller left table shows the gene names of nonvalidated DASEs that only showed a single band by RT-PCR. Genes in red show poor correlation between RNA-seq and RT-PCR PSIs, so are not validated. (D) Breakdown of correlation coefficients for the 42 tested DASEs as parameter for validation, showing a total of 67% (24 + 43) validated DASEs with correlation coefficient >0.4.
