PRPF40B regulates differential AS events (DASEs). (A) Number of cassette-exon DASEs upon KO (K562 vs. KO), in rescue WT cells (KO vs. WT rescue) and those in common between the two comparisons which are regulated by PRPF40B (AS events Rescued) (FDR < 1, ΔPSI ≥ 0.1 ≤ −0.1). (B) Number of cassette exons regulated by PRPF40B (AS events Rescued) which are (blue) or are not (red) rescued by the MDS mutations. (C) Gene Ontology of the cassette-exon DASEs regulated by PRPF40B. We noted an enrichment in genes with binding sites for the Pol II subunit POLR2A based on Chip-seq data, with histone marker H2AFZ, and which are involved in MAPK signaling cascade. (D) Network of PPIs of the cassette exons regulated by PRPF40B. Genes with same function are grouped in clusters (dotted circles) with labels. Right shows a zoomedin view of two selected clusters, ubiquitination, and Akt/MAPK1.
