PRPF40B regulates hypoxia and other relevant pathways. (A) Heatmap of DEGs with a role in hypoxia. PRPF40B-KO induces the up-regulation of hypoxic genes that are down-regulated when the expression of PRPF40B-WT gene is reintroduced in the KO cells. In many cases, overexpression of the MDS mutants also reversed the expression changes in the KO. The six DEGs selected for validation are highlighted in red. (B) Validation by real-time RT-PCR of six selected hypoxic genes with different functions as indicated. The y-axis indicates expression of the gene for each condition, relative to that on parental cells normalized to 1. (C) DEGs that are targets of KLF1, showing strong up- or down-regulation in the KO that are rescued by WT and MDS-mutant PRPF40B. (D) Homeobox (HOX) genes as DEGs regulated by PRPF40B. (E) DEGs involved in iron metabolism are mostly up-regulated upon PRPF40B-KO. (F) Cytokines and chemokines under expression control of PRPF40B.
