Usage of tissue-specific alternative TSSs maintains lnc-pou2af1 expression in a subset of adult tissues. (A) The lnc-pou2af1 locus in zebrafish. Shown are the corresponding CAGE (Nepal et al. 2013; Haberle et al. 2014), H3K4me3 ChIP-Seq (Ulitsky et al. 2011), and RNA-seq tracks from WT zebrafish. (B) The lnc-pou2af1ΔTSS mutant allele showing deletion of the sequence around the TSS (dotted, blue line). Indicated are positions of the 5′ RACE and qPCR primers and alternative TSS. Red box represents a new exon generated from the alternative TSS. (C–E) lnc-pou2af1 expression across a subset of adult WT and homozygous lnc-pou2af1ΔTSS zebrafish tissues detected by qRT-PCR. eef1α1l1 was used as a reference gene in all qRT-PCR experiments. Each dot represents an individual biological replicate. Data are presented as mean ± S.E.M.; (*) P < 0.05, (**) P < 0.01, (***) P < 0.001, n.s., not significant, unpaired t-tests.
