Effect of overexpression of sRNAs on Hfq expression in wild-type cells. (A) IT1568 cells harboring indicated plasmids were grown to A600 = ∼0.3. Protein samples equivalent to 0.025 or 0.0025 A600 units were subjected to western blotting using anti-Hfq polyclonal antibodies or anti-GroEL polyclonal antibodies, respectively. Relative Hfq levels are calculated with the pMW218 sample set to one. (B) IT1568 cells harboring indicated plasmids were grown to A600 = ∼0.3, and to A600 = ∼1.9. Protein samples equivalent to 0.025 A600 units were subjected to western blotting using anti-Hfq polyclonal antibodies. Relative Hfq levels are calculated, with the pMW218 sample at exponential phase set to one.
