ALN-F12-rescued mustard oil-induced vascular permeability in a C1INH knockdown mouse model. Female CD1 mice were subcutaneously dosed with a siRNA targeting C1INH (10 mg/kg) and ALN-F12 at different dose levels (n = 10 per group). Seven days post-administration, Evans blue dye (30 mg/kg) was given IV, and 5% mustard oil was topically applied to the right ear, followed 15 min later by a second application. The animals were euthanized 15 min after the second application of mustard oil. Both right (mustard oil-treated) and left (control) ears were collected for dye extraction (A). Livers were collected for mRNA measurements (B). Group mean ± SD are plotted. Statistical analysis in Panel A performed using two-way ANOVA with Tukey's post-hoc test; asterisks indicate statistical comparisons to mustard oil-treated ears in the PBS control group. Statistical analysis in Panel B performed using one-way ANOVA with Dunnett's post-hoc test; statistical comparisons to PBS-treated animals. For both analyses: (**) P < 0.01; (****) P < 0.0001.
