Roles of specific aminoglycoside–ribosome interactions in the inhibition of translation

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 3.
FIGURE 3.

Inhibition of EF-G-dependent translocation by Neo in polymix buffer. Shown is the extent of translocation (Fraction POST) in control and mutant ribosomes (as indicated) plotted against the concentration of Neo. Data were fit to a modified dose response equation, yielding the following micromolar IC50 values (mean ± SEM, n ≥ 3): Control, 3 ± 0.4; A1408G, 45 ± 10; ΔH69, 15 ± 3; A1408G/ΔH69, 130 ± 30.

This Article

  1. Published in Advance November 9, 2018, doi: 10.1261/rna.068460.118 RNA 25: 247-254

Current Issue

  1. August 2026, 32 (8)
  1. Current Issue
  1. Alert me to new issues of RNA