Mutually exclusive amino acid residues of L13a are responsible for its ribosomal incorporation and translational silencing leading to resolution of inflammation

In vivo association of L13a and its mutant variants with nucleolin. (A) Lysates of HEK 293T cells expressing recombinant HA-tagged L13a (WT or mutant as indicated above each lane) were subjected to immunoprecipitation with anti-HA-coated beads (or nonantibody-coated blank beads as a control) as described for Figure 3. The immunoprecipitates were run on SDS-PAGE gels and immunoblotted with antinucleolin antibody (top panel), inputs of nucleolin before immunoprecipitation (bottom panel). (B) Immunoblot with anti-HA antibody.