FIGURE 4.
Nuclear and nucleolar localization of L13a and its mutant variants. HEK 293T cells were transfected with constructs directing expression of HA-tagged L13a (WT or deletion mutants [1–148] and [149–203] or triple point mutants Lys159Ala–Arg160Ala–Lys161Ala, Val185Ala–Ile189Ala–Leu196Ala, and Arg59Ala–Lys60Ala–Lys61Ala as indicated above each set of six images). Cells were fixed 24 h post-transfection, stained with anti-HA (green), anti-nucleolin (red; a marker of the nucleolus), and DAPI (blue; to visualize nuclei), and viewed under a fluorescence microscope.
