Sequestration of microRNA-mediated target repression by the Ago2-associated RNA-binding protein FAM120A

FAM120A stabilizes its bound 3′-UTRs through G-rich motifs. (A,B) 3′ UTR reporter assay of Ago2-bound miR-294 target genes not bound by FHFAM120A or having G-rich motif-positive FHFAM120A iCLIP clusters in TT-FHAgo2 mESCs (A) and TT-FHFAM120A mESCs (B). mESCs were cultured in the absence (Dox −) or presence (2.5 μg/mL, Dox ++) of Dox for 48 h, transfected with luciferase reporter plasmids, and then subjected to luciferase reporter assays 48 h after transfection. (C) Effects of mutation of G-rich motifs on FAM120A-mediated target stabilization in TT-FHFAM120A mESCs. (WT) Wild-type, (Mut) mutant.