Sequestration of microRNA-mediated target repression by the Ago2-associated RNA-binding protein FAM120A

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FIGURE 2.
FIGURE 2.

iCLIP of FAM120A reveals binding to 3′-UTRs of mRNA and an enrichment of G-rich motifs. (A) Breakdown of RNA species bound to FHFAM120A from FHFAM120A iCLIP analysis. (B) Localization of FHFAM120A iCLIP clusters within bound mRNAs. A binding profile of untagged FAM120A iCLIP library is shown as a control. (C) Heatmap of FHFAM120A iCLIP clusters along mRNA for FHFAM120A-bound genes. (D) MEME analysis of the 20 nt sequences flanking the summits of the top 1500 FHFAM120A iCLIP clusters. (E) Hexamer enrichment analysis in FAM120A iCLIP clusters. In the middle panel, the x-axis shows the z-scores of hexamer frequency. The y-axis shows the negative log P-value of statistical significance of the hexamer enrichment above background. The right panels show a comparison of G-rich hexamers with A/T and C/G. (F) Positional enrichment of 5G + A/T motifs around the cross-link sites.

This Article

  1. Published in Advance July 9, 2019, doi: 10.1261/rna.071621.119 RNA 25: 1291-1297

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