Human angiogenin is a potent cytotoxin in the absence of ribonuclease inhibitor

Bar graphs showing the effect of tRF transfection on the viability of (A) ΔRNH1 and (B) wild-type HeLa cells. Cell viability (±95% confidence interval) after transfection of 1 or 3 µg of an RNA or DNA species. Significant cell death compared to transfection reagent (jetPRIME) are marked by asterisks, where (*) refers to P ≤ 0.05; (**) P ≤ 0.01; and (***) P ≤ 0.001. One microgram or 3 µg transfection corresponds to ∼1 or 3 µM RNA species in cell culture medium.