FIGURE 1.
Five protocols of 3′-amino-tailing of tRNA. (A) Protocols 1–5 are shown in the outlined format and each arrow indicates one reaction, where the enzyme is shown below the arrow and key reagents above the arrow. Of these, protocol 2 is the most efficient. (B) A tRNA L-shaped tertiary structure is shown, where the terminal nA nucleotide and the attached amino acid (aa) are shaded in light green and light yellow, respectively. The chemical structure of the ribose in nA is shown below, where the 3′-amino group is highlighted and its amide linkage to the amino acid is circled.
