Comparative genome analysis. (A) Phylogenetic tree of the selected 21 acetogens including psychrotolerant, mesophilic, and thermophilic acetogens. Bootstrap values are shown at nodes and low bootstrap values (<70) were clipped based on 1000 trials. The maximum-likelihood phylogenetic tree was constructed in MEGA v6.06 (Tamura et al. 2013). The 16S rRNA gene sequences, obtained from genome sequence data, are shown in bold. The accession numbers for each sequence are shown after the strain name. (B) Pairwise comparison of the average nucleotide identity based on BLAST+ (ANIb) and tetranucleotide composition (TETRA) values among the available genomes of seven acetogen species analyzed via JSpeciesWS (Richter et al. 2016). Each value is shown as a row and a column with the order of species being the same on both axes. (C) Venn diagram of orthologous gene cluster between A. bakii and A. woodii. (D) An alignment plot between the A. bakii scaffold 1 and A. woodii reference genome. This plot was generated using NUCmer version 3.1 (Kurtz et al. 2004) from the MUMMER package. The syntenic blocks, representing conserved segments, were generated using Mauve alignment with default parameters (Darling et al. 2010). All the syntenic blocks were highlighted with two distinguishable colors in this dot plot.
