Gene regulation by a glycine riboswitch singlet uses a finely tuned energetic landscape for helical switching

Targeted analysis of double mutant data. (A) In vitro transcription termination response profiles generated by SMARTT for WT and A50G/U85C. Although a mean of just 22.8 reads per glycine concentration was obtained for A50G/U85C, the data suggest this double mutant has an improved K_1/2 compared to WT. This was verified by gel. (B) In vitro transcription termination response profiles obtained by the conventional gel-based approach for WT and A50G/U85C. Filled circles indicate that glycine was used as the ligand; open circles indicate alanine. Error bars represent the standard deviation.