The binding of Class II sRNA MgrR to two different sites on matchmaker protein Hfq enables efficient competition for Hfq and annealing to regulated mRNAs

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FIGURE 7.
FIGURE 7.

Free energy profiles for the annealing of MgrR to eptB133 or ygdQ145 mRNA fragments. The free energy profiles for the competing pathways in the annealing of MgrR to eptB133 mRNA (A) or ygdQ145 mRNA (B) were computed with KinTek Explorer using simple transition state theory (see Materials and Methods). The symbols describing unbound molecules and their complexes are the same as in the legends to Figures 5 and 6 for reactions with eptB133 and ygdQ145, respectively.

This Article

  1. Published in Advance September 14, 2018, doi: 10.1261/rna.067777.118 RNA 24: 1761-1784

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