Zebrafish Slbp2 regulates the storage of histone mRNAs and proteins during zebrafish oogenesis. (A) Relative expression by qPCR detection of four replication-dependent histones in WT and Zslbp2Δ4−/− ovaries, or WT and MZslbp2Δ4−/− embryos at the two-cell, 256-cell, and high stages. ef1α was used as the control. Each bar represents mean ± SD (n = 3). Asterisks (*) indicate significant differences (P < 0.05) between WT and Zslbp2Δ4−/− ovaries, or WT and MZslbp2Δ4−/− embryos. Data were performed from three independent experiments. (B) Western blot detection of H2b and H3 proteins in WT and Zslbp2Δ4−/− ovaries, and WT and MZslbp2Δ4−/− embryos at two-cell stage and high stage. The developmental stages of embryos are marked at the bottom. (C) Relative expression by qPCR detection of three histone variants in WT and MZslbp2Δ4−/− embryos at the 256-cell stage and high stage. ef1α was used as the control. Each bar represents mean ± SD (n = 3). Asterisks (*) indicate significant differences (P < 0.05) between WT and MZslbp2Δ4−/− embryos. (D) EMSA analysis of the binding ability between Slbp2 and SL of H2a mRNA.
