The establishment of zebrafish slbp2 and slbp1 knockout mutant lines by CRISPR/Cas9. (A) The multiple amino sequence alignments of Xenopus laevis SLBPs (xSLBP1 and xSLBP2) and zebrafish Slbps (Slbp1 WT, Slbp1Δ1, Slbp2 WT, and Slbp2Δ4). The RBD domain of the SLBPs is shown by a black rectangle, and other conserved motifs are shown by a red rectangle. (B,E) The target sites of zebrafish slbps. The exons and introns are depicted as rectangular boxes and thick lines. (C,F) Sequences of WT slbps and slbps mutations. (D,G) Schematic representation of WT Slbps proteins and mutated Slbps proteins. The RNA binding domain (RBD) is indicated by the gray box. The different protein sequences between WT Slbps and mutated Slbps are shown by the blue rectangular box.
