Dynamic association of human mRNP proteins with mitochondrial tRNAs in the cytosol

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FIGURE 2.
FIGURE 2.

Conservation of YBX1 and YBX3 interaction with mt tRNAs in human and mouse cells. (A) YBX1 and YBX3 interaction with mt tRNAs in human cell lines. YBX1 and YBX3 were immunoprecipitated from extracts (Ext) of HeLa, MCF7, MRC5V1, U2OS, and VA13 cells. IP of YBX1 and YBX3 and copurification of mt tRNALys and mt tRNAPhe were monitored by western and northern blot analyses, respectively. (B) Transiently expressed Flag-tagged YBX1 interacts with endogenous mt tRNALys and mt tRNAPhe in human HeLa, MCF7, MRC5V1, U2OS, and VA13 cells. FL-YBX1 was immunoprecipitated with an anti-Flag antibody from extracts (Ext) of nontransfected (NT) and transfected (pFL-YBX1) cells. IP of FL-YBX1 and co-IP of mt tRNALys and mt tRNAPhe were monitored by western and northern blotting. (C) YBX1 interacts with mt tRNALys and mt tRNAPhe in human primary fibroblast cells. Please note that fibroblast cells do not express YBX3. (D) Mouse YBX1 binds to mt tRNAs. Mouse YBX1 (mYBX1) was immunoprecipitated from an L929 cell extract with a specific antibody, and coprecipitation of four selected mt tRNAs was tested.

This Article

  1. Published in Advance August 23, 2018, doi: 10.1261/rna.066738.118 RNA 24: 1706-1720

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