Structural basis of IMP3 RRM12 recognition of RNA

Binding affinity determination of IMP3 RRM12 with RNA. Titration of IMP3 RRM12 into CCCC (A), AAAA (B), and ACAC (C), monitored by ITC. The top panel shows titration experiment plotted as heat (µcal sec⁻¹) versus time (min), and the lower panel shows binding isotherm with the integrated heat (kcal mol⁻¹) of IMP3 RRM12 versus the molar ratio of IMP3 RRM12 to RNA. The continuous line shows the fit of the data to a one-site binding model. (D) Summary of ITC measurements of IMP3 RRM12 binding to above RNAs.