Molecular mechanism of substrate recognition and specificity of tRNAHis guanylyltransferase during nucleotide addition in the 3′–5′ direction

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FIGURE 1.
FIGURE 1.

Reaction scheme for G−1 addition to tRNAHis by eukaryotic Thg1. First, the 5′-monophosphorylated pre-tRNAHis is cleaved by RNase P, and then activated by adenylylation using ATP. Second, GTP is transferred to the activated 5′-end of pre-tRNAHis. Finally, the 5′-pyrophosphate is removed from the G−1 nucleotide to yield the monophosphorylated, G−1-containing tRNAHis that is the determinant for aminoacylation by HisRS.

This Article

  1. Published in Advance August 15, 2018, doi: 10.1261/rna.067330.118 RNA 24: 1583-1593

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