Requirement of the BP3831 transporter for growth of B. pertussis in chemically defined media. (A) B. pertussis Tohama I strain and its isogenic ΔBP3831 mutant were grown in chemically defined SS medium in the presence (diamonds and squares, respectively) or absence (triangles and circles, respectively) of cysteine. (B) B. pertussis Tohama I strain and its isogenic ΔBP3831 mutant were grown in chemically defined SS medium in the presence (diamonds and squares, respectively) or absence (triangles and circles, respectively) of proline. (C) B. pertussis Tohama I strain and its isogenic ΔBP3831 mutant were grown in chemically defined SS medium in the presence (diamonds and squares, respectively) or absence (triangles and circles, respectively) of glutamate. (D) B. pertussis Tohama I strain (diamonds) and its isogenic ΔrgtA (squares), ΔBP3831 (triangles), ΔbvgA mutant (filled circles), and ΔbvgAΔrgtA (empty circles) mutants were grown in chemically defined SS medium with the glutamate as the sole source of carbon and nitrogen (no proline). The results in all panels are representative of three experiments.
