The 2′Ome level of MIR168 in Arabidopsis thaliana, miR-16 in mouse lung tissue, and piR-31068 in human seminal plasma detected by nonlinear regression method. (A) The 2′Ome level of Arabidopsis thaliana MIR168 and synthetic methylated or unmethylated MIR168 detected by nonlinear regression method. (B) The 2′Ome level of mouse lung miR-16 and synthetic 2′Ome (methylated) or unmethylated miR-16 detected by nonlinear regression method. (C) The 2′Ome level of piR-31068 in human seminal plasma and synthetic methylated or unmethylated piR-31068 detected by nonlinear regression method. In panels A–C, the detected methylation ratios are indicated on the top of the column, while the standard curves of Ct versus methylation for MIR168, miR-16, and piR-31068 are shown in the upper right, respectively. Data are presented as Mean ± SE (n = 3 or 9 as indicated).
