RNA pairing strength is correlated with 3′ alternative cassette selection of PGRP-LC pre-mRNA. (A) Schematic diagrams of the constructs used to assess the effects of RNA pairing on alternative cassette LCx inclusion. Symbols used are the same as those in Figure 1. Predicted RNA pairing for the WT and a series of mutants (point mutations are shown in red) with the estimated equilibrium free energies (given in kcal/mol). (B) The strength of RNA pairing modulated the alternative cassette LCx inclusion according to mutation analysis. (C) The strength of intronic RNA pairing was correlated with LCx selection. (D) Schematic diagrams of the constructs used to assess the effects of RNA pairing on LCy inclusion. (E) The strength of intronic RNA pairing modulated LCy inclusion according to mutation analysis. (F) The strength of intronic RNA pairing was correlated with LCy inclusion.
