Age-dependent circRNAs in testes. (A) Paired comparisons of correlation coefficients (ρ) between the age-dependent circRNAs (ρ > 0.6) and their linear counterparts in rat testes. The ρ values were calculated between age and the expression of linear/circular transcripts using Spearman's rank correlation test. (B) The top 10 GOBP terms associated with the age-dependent circRNAs in rat testes. The P-values were calculated with Fisher's exact test. The vertical dashed line denotes the significance level of α = 0.05. (C) The circRNA abundance in mouse spermatogenic cells was categorized by distinct stages (spermatogonia, spermatocytes, and spermatids, sequentially). (D) The circRNA expression-based pathway scores of the mouse spermatogenic cells. We calculated the circRNA pathway scores for both the “cilium morphogenesis” and “spermatogenesis” GOBP terms. Higher pathway scores indicate higher overall circRNA expression for the specific GOBP terms.
