Assessment and comparison of thermal stability of phosphorothioate-DNA, DNA, RNA, 2′-F RNA, and LNA in the context of Phi29 pRNA 3WJ

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FIGURE 4.
FIGURE 4.

Strand displacement between radiolabeled single-stranded 3WJ-c and assembled homogeneous 3WJs. (A,E) LNA displaces 3WJs. (B,F) 2′-F RNA displaces 3WJs. (C,G) RNA displaces 3WJs. (D,H) DNA displaces 3WJs. (A–D) EtBr channel; (E–H) phosphorimaging.

This Article

  1. Published in Advance October 19, 2017, doi: 10.1261/rna.063057.117 RNA 24: 67-76

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