FIGURE 1.
In vitro reconstitution of human Ago2-RISC assembly by recombinant proteins. (A,B) Coomassie staining of immunopurified Ago2 and recombinant Hsp70/Hsp90 chaperone machinery proteins. (C) Target RNA cleavage assay by reconstituted human Ago2-RISC. The highest cleavage activity was observed when all five chaperone proteins were present. (D) Quantification of target cleavage in Figure 1C. The fraction target cleaved is the amount of cleaved target divided by the sum of full-length target and cleaved target. The mean ± SD from three independent experiments is shown.
