Sbp1 modulates the translation of Pab1 mRNA in a poly(A)- and RGG-dependent manner

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FIGURE 4.
FIGURE 4.

Sbp1, Pab1, and eIF4G1 interact with each other and formation of the ternary complex is RNA-dependent. IgG-pulldown assay showing that Sbp1, Pab1, and eIF4G1 interact in the presence of 5′UTRPab1 (cf. lanes 2 and 3), and the molecular interaction is disrupted by RNase treatment (cf. lanes 3 and 4). Lane 1 shows that Sbp1 and eIF4G1 do not interact with each other when Pab1 is absent.

This Article

  1. Published in Advance October 6, 2017, doi: 10.1261/rna.062547.117 RNA 24: 43-55

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