Cryo-EM structures of B. subtilis strain 168 70S ribosome (PDB: 3J9W) and E. coli 70S ribosome (PDB: 5UYL) to show glyoxal-reactive nucleotides. (A) B. subtilis 16S rRNA is shown as blue sticks, with glyoxalated nucleotides colored red. Proteins are in cartoon form and are colored yellow. (B) B. subtilis 23S rRNA is shown in cartoon form and is colored pale blue, except for nucleotides within the examined range, which are shown as sticks and are colored darker blue. Reactive nucleotides are colored red. Proteins are shown in cartoon form and are colored yellow. (C) E. coli 5S rRNA. All RNAs are shown as space-filling spheres, with 5S rRNA in blue and 23S rRNA in gray. Proteins are in cartoon form and are colored yellow. G13 and G41 are red, G81 is magenta, all reactive Cs (C19, C35–C38, and C47) are orange, and A52 is olive.
