Glyoxals as in vivo RNA structural probes of guanine base-pairing

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FIGURE 5.
FIGURE 5.

Glyoxal, methylglyoxal, and phenylglyoxal modification of rice 5.8S rRNA in vivo analyzed by denaturing PAGE of cDNAs generated by reverse transcription. Reaction conditions at buffer pH 7 or pH 9.2 for no reagent (NR), glyoxal (GX), methylglyoxal (MG), and phenylglyoxal (PG) are shown, along with dideoxy sequencing lanes. Red text indicates reactive guanines.

This Article

  1. Published in Advance October 13, 2017, doi: 10.1261/rna.064014.117 RNA 24: 114-124

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