Glyoxal, methylglyoxal, and phenylglyoxal modification of rice 5.8S rRNA in vivo. (A) Denaturing PAGE analysis of cDNAs generated after reverse transcription. PAGE with glyoxal and methylglyoxal was run on a separate gel from phenylglyoxal and is indicated by gray brackets. Reaction conditions at buffer pH 8 or pH 9.2 for no reagent (NR), glyoxal (GX), methylglyoxal (MG), and phenylglyoxal (PG) are shown, along with dideoxy sequencing lanes. Red text indicates reactive guanines, and outlined text indicates natural reverse transcriptase stops. (B) Reactive guanines mapped as red discs onto the relevant portion of rice 5.8S rRNA comparative structure. (C) Fold change of methylglyoxal and phenylglyoxal reactivity at G82, G89, and G99 relative to glyoxal reactivity at these same residues.
