RNase II regulates RNase PH and is essential for cell survival during starvation and stationary phase

Effect of membrane-anchoring domain of RNase II on rRNA degradation during starvation. WT, RNase II⁻, and RNase II (Δ19) cells were first grown in M9/glucose at 31°C to early exponential phase and then starved of glucose for 4 h at 42°C. Cells were collected by centrifugation, and total RNA was extracted and resolved by Synergel/agarose gel electrophoresis, as described in Materials and Methods. The gel was stained with ethidium bromide and visualized by UV.