N⁶-methyladenosine is required for the hypoxic stabilization of specific mRNAs

Individual mRNAs are stabilized under hypoxia in an m⁶A-dependent manner. Total RNA from HEK-293T cells was harvested after 72 h transfection with METTL3/14 siRNA (M3/14 siRNA) or negative control siRNA (Neg siRNA) and 24 h of normoxic or hypoxic conditions and half-life determined via 4sU. (#) denotes P ≤ 0.05 by paired Student's t-test between negative siRNA samples in normoxic and hypoxic conditions while (*) denotes P ≤ 0.05 by paired Student's t-test between negative and M3/14 knockdown siRNAs in the hypoxic condition. Error bars represent SEM of five experiments.