Focal accumulation of preribosomes outside the nucleolus during metaphase–anaphase in budding yeast

The formation of the extranucleolar body does not involve the same mechanisms used for tethering the rDNA and tDNA to the nuclear envelope. (A,B) Quantification of the presence of Nop7-GFP at the extranucleolar body in early mitosis cells from the indicated yeast strains. The experimental plan was the same as the one outlined in Figure 1A for wild-type cells, except in the case of the nup159-1 cells, which were arrested in G₁ with α-factor at 24°C and then shifted to 37°C upon release from the G₁ arrest.