FIGURE 5.
Pan3L and Pan3S knockdowns exhibit differential effects on P-body abundance. Human U2OS cells were transfected with control siRNA (control KD), Pan3L-specific siRNA (Pan3L KD), Pan3S-specific siRNA (Pan3S KD), or both Pan3 siRNAs (Pan3S+L KD). Immunofluorescence microscopy was used to visualize markers for total P-bodies (TNRC6 in A,B; green or Rck/p54 in A; red) or for GW/P-bodies (GW220 in B; red) as described in Materials and Methods. RT-PCR and gel electrophoresis were used to assess the Pan3 knockdown efficiency; GAPDH served as the loading control. Scale bars, 50 µm.
