FIGURE 2.
Effects of Pan3 domain truncations on Pan3–PABP and Pan3–Pan2 interactions. (A) Schematic diagram showing various domain truncations of Pan3S and Pan3L. (B,C) Coimmunoprecipitation (Co-IP) and Western blot analyses showing the effects of Pan3 domain truncations on HA-Pan3 isoforms’ abilities to pull down endogenous PABP and Pan2. NIH3T3 cells were transfected with plasmids coding for the indicated epitope-tagged proteins. GAPDH served as the negative control for co-IP. The amount of each “Input” sample loaded onto the gel was 5% of the amount of the corresponding lysate used for IP.
