The C-terminal dsRNA-binding domain of Drosophila Dicer-2 is crucial for efficient and high-fidelity production of siRNA and loading of siRNA to Argonaute2

Both N-terminal helicase domain and C-terminal dsRBD of Dicer-2 are important for siRNA loading to Argonaute2 in ovary lysate in vitro. (A) Representative gel image of in vitro siRNA loading assay using ovary lysate. (B) Quantification of the signals in the gels normalized to the background signals in the ovary lysate of dicer-2^null without any rescue gene (negative control). Data are mean ± SD for four independent trials. (*) P-value <0.05.